Movie 6.

SR-SIM sections of MII spindle poles in control WT and Tg (PCNT-depleted) oocytes following AURKA inhibition. Z-stacks of structurally illuminated super-resolved images (91 um intervals) acquired on the ELYRA S1 SR-SIM microscope using NIS Elements software. Ovulated MII oocytes from WT and Tg mice were fixed after a 4h culture in media supplemented with MLN8237 (500nM) to inhibit AURKA, then immunolabeled to detect TACC3 (green), PCNT (red).