Movie 1.
Kv2.1 clusters are detergent insoluble structures. HEK cells expressing GFP-Kv2.1 162 were imaged at 1.1Hz with the focal plane at the basal surface following the addition of lysis buffer 163 (50 mM Tris Base, 150 mM NaCl, 1 mM EDTA and 1% Triton X-100, pH 7.2) at 20°C. At 164 1.5 min after this detergent addition the membrane and nonclustered GFP-Kv2.1 begin to 165 solubilize. The GFP-Kv2.1 clusters first aggregate and then assume a globular shape but do not 166 solubilize even after 5 min. Non-solubilized GFP-Kv2.1 aggregates can be seen both diffusing 167 through the solution and adhered to the substrate.
Kv2 channel–AMIGO β-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.256339