Movie 3

Odor application induces transient elevation of GCaMP6f fluorescence in knobs of mouse OSNs. Ectopically expressed GCaMP6f was used to report stimulus-evoked activity in mouse OSNs. Live en face imaging of acutely dissected olfactory epithelium reported change of GCaMP6f fluorescence in the dendritic knobs of Inpp5e^osnKO OSNs. A 100-ms pressure-pulse was applied from the micropipette filled with ACSF and added odor mix. Total of 5 pulses was applied with 60-s intervals in between. Real time rate is 25 frames per second.