Movie 1.

Polymerization of recombinant human septin octamers-9_i3. Optical sectioning (z-stack with a Δz interval of 0.5 μm) in the bulk of a flow channel depicting SEPT2-msfGFP human septin octamer-9_i3 polymerized at 0.3 μM (100% GFP-septins) by dilution into low-salt (50 mM KCl) buffer. Spinning disk fluorescence images displayed at 5 frames per second. Related to Fig. S2 B.

Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms

Francois Iv, Carla Silva Martins, Gerard Castro-Linares, Cyntia Taveneau, Pascale Barbier, Pascal Verdier-Pinard, Luc Camoin, Stéphane Audebert, Feng-Ching Tsai, Laurie Ramond, Alex Llewellyn, Mayssa Belhabib, Koyomi Nakazawa, Aurélie Di Cicco, Renaud Vincentelli, Jerome Wenger, Stéphanie Cabantous, Gijsje H. Koenderink, Aurélie Bertin, and Manos Mavrakis

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258484