Movie 2.

Reconstitution of single actin filaments. Time-lapse sequence (Δt interval of 0.5 s) at the surface of a PLL-PEG passivated glass coverslip showing single fluctuating actin filaments at 1 μM. G-actin was polymerized in the presence of Alexa Fluor 568-phalloidin. Spinning disk fluorescence images displayed at 5 frames per second. A still image from this time lapse sequence is shown in Fig. 4A.