Movie 2.
Reconstitution of single actin filaments. Time-lapse sequence (Δt interval of 0.5 s) at the surface of a PLL-PEG passivated glass coverslip showing single fluctuating actin filaments at 1 μM. G-actin was polymerized in the presence of Alexa Fluor 568-phalloidin. Spinning disk fluorescence images displayed at 5 frames per second. A still image from this time lapse sequence is shown in Fig. 4A.
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258484