Movie 3.

Actin filament cross-linking by recombinant human septin octamers-9_i1. Time-lapse sequence (Δt interval of 0.5 s) at the surface of a PLL-PEG passivated glass coverslip showing cross-linked actin filaments (at 1 μM) in the presence of SEPT2-msfGFP human septin octamer-9_i1 at 0.3 μM (20% GFP-septins). G-actin was polymerized in the presence of Alexa Fluor 568-phalloidin. The actin channel is shown. Spinning disk fluorescence images, using an inverted grayscale, are displayed at 5 frames per second. A still image from this time lapse sequence is shown in Fig. 4C.

Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms

Francois Iv, Carla Silva Martins, Gerard Castro-Linares, Cyntia Taveneau, Pascale Barbier, Pascal Verdier-Pinard, Luc Camoin, Stéphane Audebert, Feng-Ching Tsai, Laurie Ramond, Alex Llewellyn, Mayssa Belhabib, Koyomi Nakazawa, Aurélie Di Cicco, Renaud Vincentelli, Jerome Wenger, Stéphanie Cabantous, Gijsje H. Koenderink, Aurélie Bertin, and Manos Mavrakis

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258484