Movie 2.

JIP4 localizes in subdomains on RAB5 positive macropinosomes. Shown is a macropinocytosing RPE1 cell with mNeonGreen-JIP4 (pseudocolored green) and mCherry-Rab5 (pseudocolored magenta). JIP4 localizes to dynamic subdomains as the macropinosome acquires RAB5.

JIP4 is recruited by the phosphoinositide-binding protein Phafin2 to promote recycling tubules on macropinosomes

Kia Wee Tan, Viola Nähse, Coen Campsteijn, Andreas Brech, Kay Oliver Schink, and Harald Stenmark

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258495