Movie 4.

VAMP3 exits the macropinosome in JIP4-positive tubules. Shown is a macropinocytosing RPE1 cell with mNeonGreen-JIP4 (pseudocolored green) and VAMP3-mCherry (pseudocolored magenta). VAMP3 leaves the macropinosome in tubulating membrane structures that are positive for JIP4. The top macropinosome appears to deplete of VAMP3.

JIP4 is recruited by the phosphoinositide-binding protein Phafin2 to promote recycling tubules on macropinosomes

Kia Wee Tan, Viola Nähse, Coen Campsteijn, Andreas Brech, Kay Oliver Schink, and Harald Stenmark

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258495