Movie 10

Neutrophil-specific rac2 knockout abolished the oscillation between the front and rear of active Rac in neutrophils.
The video shows the subcellular localization of Rac-FRET, of which the YFP/CFP fluorescence ratio indicates the location of active Rac in neutrophils of 3 dpf Tg(lyzC:Cas9, cry:GFP)^pu26 zebrafish larvae injected with plasmids containing control or rac2 sgRNAs. Scale bar: 10 μm.