Movie 11
Neutrophil-specific rac2 knockout induced stable F-actin changes in neutrophils.
The video shows neutrophils expressing GFP-UtrCH, which labels stable F-actin, along with control or rac2 sgRNAs in 3 dpf Tg(lyzC:Cas9, cry:GFP)pu26 zebrafish larvae. Cytoplasm is labeled with mCherry. Scale bar: 20 μm.
A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574