Movie 12
Neutrophil-specific rac2 knockout abrogated the generated actin stress at the front and the back.
The video shows neutrophils expressing AcpA-FRET along with control or rac2 sgRNAs in 3 dpf Tg(lyzC:Cas9, cry:GFP)pu26 zebrafish larvae. The ratiometric AcpA-FRET signals report the actin force in neutrophils. Scale bar: 100 μm.
A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574