Movie 13

Tracked movies of migrating neutrophils in the head mesenchyme transiently expressing control or rac2 sgRNAs.
The video shows the motility of neutrophils in 3 dpf Tg(ubb:cas9, cry:GFP)xt48 zebrafish larvae injected with plasmids carrying Ribozyme-processing machinery along with the control or rac2 sgRNAs. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.