Movie 14

Tracked movies of migrating neutrophils in the head mesenchyme of zebrafish transiently expressing the neutrophil specific RFP with or without control or rac2 sgRNAs.
The video shows the motility of neutrophils in 3 dpf wide-type AB zebrafish larvae transiently expressing RFP with or without control sgRNA or rac2 sgRNA in neutrophils. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 4 fish each group are shown. Scale bar: 100 μm.

A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish

Yueyang Wang, Alan Y. Hsu, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, GuangJun Zhang, David M. Tobin, and Qing Deng

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574