Movie 2

Tracked movies of neutrophil motility in the head mesenchyme of the control and rac2 knockout stable lines.
We generated stable lines by crossing Tg(lyzC:Cas9, cry:GFP)pu26 with Tg(u6a/c: ctrl sgRNA, lyzC:GFP)pu27 or Tg(u6a/c: rac2 sgRNA, lyzC:GFP)pu28. The video shows the motility of neutrophils in 3 dpf zebrafish offspring larvae. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.

A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish

Yueyang Wang, Alan Y. Hsu, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, GuangJun Zhang, David M. Tobin, and Qing Deng

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574