Movie 3
Tracked movies of neutrophil motility in the head mesenchyme of the no-guide control, control and rac2 sgRNAs stable lines.
The video shows the motility of neutrophils in 3 dpf zebrafish larvae of Tg(lyzC:GFP), Tg(u6a/c: ctrl sgRNA, lyzC:GFP)pu27 or Tg(u6a/c: rac2 sgRNA, lyzC:GFP)pu28. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.
A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574