Movie 3

Tracked movies of neutrophil motility in the head mesenchyme of the no-guide control, control and rac2 sgRNAs stable lines.
The video shows the motility of neutrophils in 3 dpf zebrafish larvae of Tg(lyzC:GFP), Tg(u6a/c: ctrl sgRNA, lyzC:GFP)pu27 or Tg(u6a/c: rac2 sgRNA, lyzC:GFP)pu28. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.

A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish

Yueyang Wang, Alan Y. Hsu, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, GuangJun Zhang, David M. Tobin, and Qing Deng

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574