Movie 5

Tracked movies of neutrophil motility in the head mesenchyme transiently expressing rac2-R-WT, rac2-R-DN, or rac2-R-CA.
The video shows the motility of neutrophils in 3 dpf Tg(lyzC:Cas9, cry:GFP)^pu26 zebrafish larvae injected with plasmids carrying rac2 sgRNAs along with rac2-R-WT, rac2-R-DN, or rac2-R-CA. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.