Movie 6

Migrating neutrophils in the head mesenchyme of the rac2-CA and the rac2-CA lines.
The video shows the motility of neutrophils in 3 dpf Tg(lyzC:rac2-WT-2a-mcherry)pu30 or Tg(lyzC:rac2-CA-2a-mcherry)pu29 zebrafish larvae. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.

A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish

Yueyang Wang, Alan Y. Hsu, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, GuangJun Zhang, David M. Tobin, and Qing Deng

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574