Movie 7

Tracked movies of migrating neutrophils in the head mesenchyme transiently expressing control or cdk2 sgRNAs.
The video shows the motility of neutrophils in 3 dpf Tg(lyzC:Cas9, cry:GFP)pu26 zebrafish larvae injected with plasmids carrying control or cdk2 sgRNAs. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.

A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish

Yueyang Wang, Alan Y. Hsu, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, GuangJun Zhang, David M. Tobin, and Qing Deng

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574