Movie 8

Transient expression of cdk2-R-WT, not cdk2-R-DN, restored cell motility in cdk2-deficient neutrophils.
The video shows the motility of neutrophils in 3 dpf Tg(lyzC:Cas9, cry:GFP)^pu26 zebrafish larvae injected with plasmids containing cdk2 sgRNAs along with cdk2-R-WT or cdk2-R-DN. Videos were recorded for 30 min with 1 min interval. Representative videos from n = 3 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.