Movie 9

Neutrophil-specific rac2 knockout lead to deficiency in the front-to-rear localization of Rac in neutrophils.
The video shows the subcellular localization of PBD-GFP, which marks the location of Rac in neutrophils of 3 dpf Tg(lyzC:Cas9, cry:GFP)pu26 zebrafish larvae injected with plasmids containing control or rac2 sgRNAs. Cytoplasm is labeled with mCherry. Scale bar: 20 μm.

A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish

Yueyang Wang, Alan Y. Hsu, Eric M. Walton, Sung Jun Park, Ramizah Syahirah, Tianqi Wang, Wenqing Zhou, Chang Ding, Abby Pei Lemke, GuangJun Zhang, David M. Tobin, and Qing Deng

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258574