Movie 2.

Live cell imaging of A1.ST cells plated on anti-CD3 coated glass wells. β1-deficient A1.ST cells were stimulated on anti-CD3 coated glass substrates. SLP-76.TRT was imaged every 2 seconds for 150 frames (5 min). Movies play at 0.06 s frame^-1, yielding a 33.3x compression rate. Scale bar is 5 μm.