Movie 2.

Dual-tagged APOE3 transiently over-expressed in ST14A cells. Dual-tag fluorescent APOE with quenching of green SepHluorin over time as APOE3 enters acidic compartments. Imaging was performed using Incucyte live cell analysis. Quantification in Fig. 5B.

Isoform-dependent lysosomal degradation and internalization of apolipoprotein E requires autophagy proteins

Gianna M. Fote, Nicolette R. Geller, Nikolaos E. Efstathiou, Nathan Hendricks, Demetrios G. Vavvas, Jack C. Reidling, Leslie M. Thompson, and Joan S. Steffan

J Cell Sci 2022. 135:None-None; doi: 10.1242/jcs.258687