Movie 1.

Neutrophil transmigration through the endothelial wall of the BVOAC in high resolution over time. The video is a blended projection of 3D imaging over time of neutrophils extravasating from the lumen of the BVOAC, through the vessel wall and into the collagen matrix surrounding the vessel. The video rotates the scene to give an overview of the red neutrophils in the lumen of the vessel on top of the green endothelial cell layer. Green blobs inside the lumen are detached and rounded up HUVEC. At 10 seconds into the video the experiment starts playing and 2 red neutrophils start poking through the vessel wall after which the time is paused and the scene is rotated to show the outside of the endothelial layer with the neutrophils poking through. The scene then changes back to inside the lumen and resumes playing at 18 seconds showing the neutrophils both crossing the barrier layer until finally they have completely crossed the endothelium and are seen migrating at the basolateral side of the endothelium. Time elapsed in the experiment is shown in the bottom right corner of the video and the adaptive scale bar is shown in the bottom left of the video.