Movie 2.

Data of experiment shown in supplemental video 1 with rendered surfaces to further reveal details of the TEM event. The video shows the raw data in a MIP projection and rotates the scene 360 degrees first before zooming in on the location where 2 neutrophils sequentially migrate at the same junction. The rendered volumes are projected on top of the MIP projection of the data at 9 seconds into the video, to clarify outlines of the EC and the 2 neutrophils focussed in this rendering. The video then plays the experiment over time until 17 seconds where the neutrophil is poking through the endothelial layer. The video stops the time and rotates to show the leading edge of the neutrophil in the matrix outside of the endothelial barrier. The video afterwards resumes displaying the experiment over time. Time elapsed in the experiment is shown in the bottom right corner of the video and the adaptive scale bar is shown in the bottom left of the video.