Movie 3.

Neutrophils migrating out of the BVOAC lumen into the collagen matrix imaged in 3D over one hour displayed from a side view. This experiment shows the raw data of red labelled neutrophils migrating out of the CellTracker green stained HUVEC vessel rendered in transparent green. HUVEC were treated overnight with TNF-α to induce inflammation. To capture as many neutrophils as possible the bottom part of the vessel and a large part of the collagen matrix were imaged. To illustrate the path travelled by a single neutrophil, the track is shown in a colour coded line which represents the time passed in the experiment. Scale bar for size is shown in the left bottom corner of the video and time including colour legend is shown in the bottom right of the video.

Transendothelial migration induces differential migration dynamics of leukocytes in tissue matrix

Abraham C. I. van Steen, Lanette Kempers, Rouven Schoppmeyer, Max Blokker, David J. Beebe, Martijn A. Nolte, and Jaap D. van Buul

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258690