Movie 5.

Neutrophils migrating out of the BVOAC lumen into the collagen matrix under the influence of c5a imaged in 3D over one hour. This experiment shows the raw data of red labelled T-cells migrating out of the CellTracker green stained HUVEC vessel rendered in transparent green. The experiment is displayed from a top view with the vessel directed horizontally and the matrix inlets located to the top and bottom of this view. A C5a gradient was created in this BVOAC by adding c5a to one of the two matrix inlets of the device. This inlet is located towards the bottom side of this video. To illustrate the path travelled by a single neutrophil the track is shown in a colour coded line which represents the time passed in the experiment. The vast majority of neutrophils migrates towards the C5a gradient for the first half of the video after which this directionality is lost and the neutrophils assume random directionality. This is also reflected in the highlighted track. Scale bar for size is shown in the left bottom corner of the video and time including colour legend is shown in the bottom right of the video.