Movie 10.

Visualization of active Rho at the plasma membrane in a cross section. Cross section of a lattice light sheet time lapse movie of a HUVEC stably expressing dimericTomato-2xrGBD sensor (upper panel, LUT = mpl-magma) and mTq2-CaaX (lower panel, grey), stimulated with 2,5 μM nocodazole 10 min prior to the imaging.

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor

Eike K. Mahlandt, Janine J. G. Arts, Werner J. van der Meer, Franka H. van der Linden, Simon Tol, Jaap D. van Buul, Theodorus W. J. Gadella, and Joachim Goedhart

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258823