Movie 12.
Visualization of active Rho in a contracting endothelial cell. TIRF microscopy time lapse movie of a BOEC stably expressing the dimericTomato-2xrGBD sensor (left, LUT = mpl-magma) and mTq2-CaaX (right, grey) stimulated with 1 U/ml human α-thrombin 5 min prior imaging. The time stamper represents min:s.
Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258823