Movie 12.

Visualization of active Rho in a contracting endothelial cell. TIRF microscopy time lapse movie of a BOEC stably expressing the dimericTomato-2xrGBD sensor (left, LUT = mpl-magma) and mTq2-CaaX (right, grey) stimulated with 1 U/ml human α-thrombin 5 min prior imaging. The time stamper represents min:s.

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor

Eike K. Mahlandt, Janine J. G. Arts, Werner J. van der Meer, Franka H. van der Linden, Simon Tol, Jaap D. van Buul, Theodorus W. J. Gadella, and Joachim Goedhart

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258823