Movie 15.

Visualization of active Rho during transendothelial migration. Spinning disk time lapse of a transendothelial migration event with cbBOECs grown in to a monolayer, expressing the dimericTomato-2xrGBD sensor (upper panel) and the membrane marker mTurquoise2-CaaX (middle panel). The transmigrating neutrophil is visible in the transmission panel. The time stamper represents min:s. LUT = mpl-inferno.

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor

Eike K. Mahlandt, Janine J. G. Arts, Werner J. van der Meer, Franka H. van der Linden, Simon Tol, Jaap D. van Buul, Theodorus W. J. Gadella, and Joachim Goedhart

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258823