Movie 15.

Visualization of active Rho during transendothelial migration. Spinning disk time lapse of a transendothelial migration event with cbBOECs grown in to a monolayer, expressing the dimericTomato-2xrGBD sensor (upper panel) and the membrane marker mTurquoise2-CaaX (middle panel). The transmigrating neutrophil is visible in the transmission panel. The time stamper represents min:s. LUT = mpl-inferno.