Movie 3.

Direct comparison of dimericTomato-2xrGBD sensor and mTurquoise2-1xrGBD sensor in one HeLa cell. Spinning disk time lapse movie of a HeLa cell expressing H1R (not shown) and CMVdel-dimericTomato-2xrGBD (magenta) and CMVdel-mTurquoise2-1xrGBD (grey) stimulated with 100 μM histamine. The time stamper represents min:s.

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor

Eike K. Mahlandt, Janine J. G. Arts, Werner J. van der Meer, Franka H. van der Linden, Simon Tol, Jaap D. van Buul, Theodorus W. J. Gadella, and Joachim Goedhart

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258823