Movie 5.

AHD+PH Rho sensor localizing in a punctate manner in an endothelial cell. Spinning disk time lapse movie of a HUVEC expressing the eGFP-AHD+PH sensor, stimulated with 1 U/ml human α-thrombin.

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor

Eike K. Mahlandt, Janine J. G. Arts, Werner J. van der Meer, Franka H. van der Linden, Simon Tol, Jaap D. van Buul, Theodorus W. J. Gadella, and Joachim Goedhart

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258823