Movie 6.

Membrane specific activation of Rho detected with the dimericTomato-2xrGBD sensor. Spinning disk time lapse movie of a HeLa cells expressing FRB anchored to the membrane (grey), FKBP-p63-DH (not shown) and the dimericTomato-2xrGBD sensor (mpI-inferno) stimulated with 100 nM rapamycin. The time stamper represents min:s.

J Cell Sci ; doi: 10.1242/jcs.258823