Movie 8.

Golgi specific activation of Rho detected with the dimericTomato-2xrGBD sensor. Spinning disk time lapse movie of a HeLa cells expressing FRB anchored to the Golgi (grey), FKBP-p63-DH (not shown) and the dimericTomato-2xrGBD sensor (mpI-inferno) stimulated with 100 nM rapamycin. The time stamper represents min:s.

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor

Eike K. Mahlandt, Janine J. G. Arts, Werner J. van der Meer, Franka H. van der Linden, Simon Tol, Jaap D. van Buul, Theodorus W. J. Gadella, and Joachim Goedhart

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258823