Movie 9.

Visualization of active Rho at the basolateral plasma membrane of an endothelial cell. TIRF microscopy time lapse movie of a BOEC stably expressing the dimericTomato-2xrGBD sensor (left, LUT = mpl-magma) and mTq2-CaaX (middle, grey), stimulated with 1 U/ml human รก-thrombin. The right shows a binary image representing the cell area. The time stamper represents min:s.

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor

Eike K. Mahlandt, Janine J. G. Arts, Werner J. van der Meer, Franka H. van der Linden, Simon Tol, Jaap D. van Buul, Theodorus W. J. Gadella, and Joachim Goedhart

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258823