Movie 9.

Visualization of active Rho at the basolateral plasma membrane of an endothelial cell. TIRF microscopy time lapse movie of a BOEC stably expressing the dimericTomato-2xrGBD sensor (left, LUT = mpl-magma) and mTq2-CaaX (middle, grey), stimulated with 1 U/ml human á-thrombin. The right shows a binary image representing the cell area. The time stamper represents min:s.