Inheritance of vacuoles and mitochondria into small buds Maximum intensity projection movie from 3D time lapse epifluorescence imaging of a cell endogenously expressing a Vph1-GFP vacuole marker (green) and a Cit1-mCherry mitochondrial marker (magenta). The cell outline from bright field imaging is in gray. The movie is 26 frames long with 1 minute separating each frame, played back at 15 frames per second.