Inheritance of vacuoles and mitochondria in hydroxyurea-treated cells Maximum intensity projection movie from 3D time lapse epifluorescence imaging of a cell endogenously expressing a Vph1-GFP vacuole marker (green) and a Cit1-mCherry mitochondrial marker (magenta), treated with hydroxyurea to prevent S-phase completion. The cell outline from bright field imaging is in gray. The movie is 23 frames long with 2 minute separating each frame, played back at 15 frames per second.