Movie 2.

Related to Figure 5. Recording of co-internalisation of keratin filaments with whole desmosomes of HGF-treated MDCK cells. Time-lapse confocal microscopy movie of scattering MDCK cells expressing either Desmocollin 2a tagged with YFP (Dsc2a, green) or keratin 18 tagged with mCherry (KRT18, magenta). Images were acquired every 15 sec for 7 min, starting 4 h after addition of HGF (40 ng/ml). Scale bar indicates 5 μm. Arrows indicate internalised desmosomes with keratin filaments from the adjacent cell.

Desmosome dualism – most of the junction is stable, but a plakophilin moiety is persistently dynamic

Judith B. Fülle, Henri Huppert, David Liebl, Jaron Liu, Rogerio Alves de Almeida, Bian Yanes, Graham D. Wright, E. Birgitte Lane, David R. Garrod, and Christoph Ballestrem

J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.258906