Movie 2.

2xDendra-IFT54 speckles pause at the tip In the ift54-2 2xDendra-IFT54 a subset of the fusion protein auto-converts from the green emitting into the red-emitting Dendra form allowing to visualizes individual IFT54 speckles during turnaround at the tip. The star marks the time points when Dendra^RED-IFT54 speckles approach the tip. The corresponding kymograms are shown at the bottom. The movie was recorded at 20fps and the timer displays seconds.