Movie 3.
Photogate showing fragmentation of an mNG-IFT54 train Single color photogate experiment showing a large anterograde mNG-IFT54 train fragmenting into eight retrograde trains. The overexposed frames were blackened and the corresponding kymogram is shown on the right. The movie was recorded at 10fps and the timer displays seconds.
In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.259010