Movie 5.
IFT140-sfGFP and mSc-IFT54 largely remain associated during tip turn around 2-color photogate experiment using the ift140-1IFT140-sfGFP ift54-2 mSc-IFT54 double-mutant-double-rescue strain. The overexposed frames were blackened. The corresponding kymograms are shown at the bottom. The movie was recorded at 10fps and the timer displays seconds.
In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip
J Cell Sci 2021. 134:None-None; doi: 10.1242/jcs.259010