Movie 2.

Imaging-based protease protection assay with HobbitΔC82-GFP. Live-cell time-lapse imaging of cytosolic mTagBFP2 (cyan), HobbitΔC82-GFP (green), and KDEL-RFP (red) in an imaging-based protease protection assay. The selective detergent digitonin, which permeabilizes the plasma membrane but not intracellular membranes, was added at timestamp 1 min 45.964512 s, and proteinase K was added at timestamp 3 min 47.512041 s. Still images from this time-lapse are pictured in Fig. S3C.