Movie 6.

Co-localization and co-trafficking of AXL-Halo, SPHK2-GFP and flotillin 2-mCherry in one MCF10AF1F2 cell. Movie acquired by spinning disk confocal microscopy of one MCF10AF1F2 cell that co-expresses flotillin 2-mCherry (red), SPHK2-GFP (green) and AXL-Halo (blue) (labeled with Halo-tag-Janelia Fluor 646) (1 frame/10 s. Scale bar: 10 μm).