Movie 7.

Flotillin 2-mCherry/SPHK2-GFP co-endocytosis in MCF10AF1F2 cells. Movie of the boxed area shown in figure 5E (upper panel) acquired by spinning disk confocal microscopy of one MCF10AF1F2 cell that co-expresses flotillin 2-mCherry and SPHK2-GFP (1 frame/2 s). At 34s, arrows indicate sites of flotillin 2-mCherry and SPHK2-GFP co-accumulation at the plasma membrane followed by endocytosis of a vesicle positive for both proteins. (Scale bar: 2 μm).