Movie 8.

Flotillin 1-mCherry/SPHK2-GFP co-endocytosis in MDA-MB-231 cells. Movie of the boxed area shown in figure 5E (lower panel) acquired by spinning disk confocal microscopy of one MDA-MB-231 cell that co-expresses flotillin 1-mCherry and SPHK2-GFP. The arrow indicates the site of flotillin 1-mCherry and SPHK2-GFP co-accumulation at the plasma membrane followed by endocytosis of one vesicle positive for both proteins (1 frame/1 s). (Scale bar: 2 μm).