Movie 9.

Inhibition of SPHK2 catalytic activity induces its delocalization from flotillin-positive endosomes. One MCF10AF1F2 cell that co-expresses SPHK2-GFP was imaged by spinning disk confocal microscopy immediately after addition of Opaganib (50 μM) (1 frame/30 s. Scale bar: 10 μm). The disappearance of the SPHK2-GFP signal in flotillin 2-mCherry-positive vesicles is clearly visible after 8 min and lasted for the rest of the movie (up to 28.5 min).