Movie 3.

Lysosome dynamics during macropinocytosis in WT and Rab7b-KO DCs WT and Rab7b-KO LPS-DCs stained with AF594-WGA to label lysosomes (cyan) were loaded in 5 x 8 μm micro-fabricated channels. After 16 hours, the channels were filled with 10 kDa 647-Dextran (magenta), and 30 minutes later the cells were imaged at imaged at 1-minute intervals. Scale bar: 10 μm.