Movie 1.

Cofilin is dispensable for cytoplasmic F-actin dynamics in GV oocytes and during NEBD. Time-lapse confocal imaging (one image every 30 sec) of F-actin networks in GV oocytes expressing EGFP-UtrCH together with catalytically-inactive LIMK1^D460A (Control, left), or LIMK1 (right). Spontaneous meiosis resumption was triggered by washing off milrinone, leading to nuclear envelope breakdown. Chromosomes are labeled with Sir-DNA (magenta). Confocal section thickness is 1 μm.