Movie 2.

Cytoplasmic F-actin is absent in LIMK1-expressing MI oocytes. Time-lapse confocal imaging (one image every 30 sec) of F-actin networks in oocytes expressing EGFP-UtrCH. Left to right: uninjected MI oocyte (Control), LIMK1-expressing MI and LIMK1^D460A-expressing MI oocytes. Chromosomes are labeled with Sir-DNA (magenta). Confocal section thickness is 1 μm.