Movie 3.

Arp2/3 inhibition exacerbates microvillar actin elongation. Confocal z-stack of a fixed LIMK1-expressing MI oocyte labeled for F-actin with Alexa Fluor 568-phalloidin. CK-666 (100 μM) was added to the culture medium at the onset of meiosis resumption (milrinone wash). Chromosomes are labeled with TO-PRO-3 (magenta). Confocal section thickness is 1 μm. The outermost layer shows a weak labeling of transzonalprojections crossing the zona pellucida.