F-actin dynamics in MII and LIMK1-expressing MII oocytes. Time-lapse confocal imaging (one image every 30 sec) of F-actin networks in a control MII oocyte expressing LIMK1-D460A (left) and an MII oocyte expressing LIMK1 (right). Oocytes also express EGFP-UtrCH to label actin filaments. Confocal section thickness is 1 μm.